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Sorter

BD Influx:
Five solid state lasers (355nm, 457nm, 488 nm, 561 nm and 642nm) with the Small Particle Option (SPO) allow simultaneous measurement and high speed sorting of fifteen parameters, including two light scatters – forward scatter (FSC) and side scatter (SSC) and thirteen fluorescence parameters
  • Sort rates up to 30,000/second
  • Small Particle Option (SPO) with resolution greater than 0.2mm
  • Circulating water bath option allows cooling or heating of sample and collection tubes
  • PC workstation with WindowsÒ XP Professional and Spigot 6.1.4 software
  • Data backup – USB, CD burner
Applications:
  • Sterile sorting of stem cells, subpopulations of GFP, mcherry, DSred transfected cells, micro particles, bacteria, etc
  • Sort up to four populations simultaneously into tubes (micro-tubes up to 50mL)
  • Sort defined cell numbers into multi-well plates (6, 24, 48, 96, 384), PCR tubes or onto slides and filters
Compatible fluorophores, partial list
 
Laser
Filter
Fluorophores
488nm (200 mW)
520/35
FITC, GFP, Alexa FluorÒ488, SYBR Green
524/27
YFP
692/40
PerCP, PerCP-Cy5.5
561nm (75 mW)
593/40
PE, RFP, DSRed, TRITC, Cy3
624/40
PI, PE-TR, mcherry
750 LP
PE-Cy7
642nm (50 mW)
670/30
APC, Cy5, DRAQ5
750 LP
APC-Cy7
457nm (300 mW)
480/40
CFP
513/17
GFP FRET
542/27
YFP FRET
355nm (100 mW)
460/50
Hoechst 33342 (Blue), DAPI
670/30
Hoechst 33342 (Red)