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Bench Top Cytometer

BD FACS Calibur:
Dual laser (488nm and 635nm) bench top flow cytometer capable of the simultaneous measurement of six parameters, including two light scatters – forward scatter (FSC) and side scatter (SSC) and four fluorescence parameters
  • Preset protocols available for data acquisition and analysis
  • Doublet Discrimination Module (DDM) for DNA cell cycle analysis
  • MACPro workstation with OS X 10.5.3 and CellQuest Pro 6.0 software
  • Data backup – USB, CD burner
Applications:
  • Single and multi-color immunophenotyping
  • DNA cell cycle analysis
  • Fluorescent protein detection and monitoring i.e., Green fluorescent protein (GFP)
  • Analysis of reactive oxygen species (ROS), cell viability, intracellular molecules, etc
Compatible fluorophores, partial list
 
Laser
Fluorescence Channel
Filter
Fluorophores
488nm Argon
FL1
530/30
Fitc, Alexa FluorÒ488, SYBR Green, Green Fluorescent Protein (GFP)
FL2
585/42
PE, Propidium Iodide (PI), Cy3
FL3
650 LP
PE-Cy5, PerCP, PE-Cy7, PerCP-Cy5.5, 7-AAD
635nm Red Diode
FL4
661/16
APC, Alexa FluorÒ647, DRAQ5