BD FACS Calibur:
Dual laser (488nm and 635nm) bench top flow cytometer capable of the simultaneous measurement of six parameters, including two light scatters – forward scatter (FSC) and side scatter (SSC) and four fluorescence parameters
- Preset protocols available for data acquisition and analysis
- Doublet Discrimination Module (DDM) for DNA cell cycle analysis
- MACPro workstation with OS X 10.5.3 and CellQuest Pro 6.0 software
- Data backup – USB, CD burner
Applications:
- Single and multi-color immunophenotyping
- DNA cell cycle analysis
- Fluorescent protein detection and monitoring i.e., Green fluorescent protein (GFP)
- Analysis of reactive oxygen species (ROS), cell viability, intracellular molecules, etc
Compatible fluorophores, partial list
Laser |
Fluorescence Channel |
Filter |
Fluorophores |
488nm Argon |
FL1 |
530/30 |
Fitc, Alexa FluorÒ488, SYBR Green, Green Fluorescent Protein (GFP) |
FL2 |
585/42 |
PE, Propidium Iodide (PI), Cy3 |
FL3 |
650 LP |
PE-Cy5, PerCP, PE-Cy7, PerCP-Cy5.5, 7-AAD |
635nm Red Diode |
FL4 |
661/16 |
APC, Alexa FluorÒ647, DRAQ5 |