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Oncologic Imaging

Frank Wuest

Associate Professor
Oncologic Imaging
Department of Oncology
University of Alberta
Cross Cancer Institute
11560 University Ave
Edmonton, Alberta T6G 1Z2
Tel: 780.989.8150

Profile

The Dianne and Irving Kipnes Chair in Radiopharmaceutical Sciences
AHFMR Senior Scholar
 

Research interests:

My research is embedded in the multidisciplinary field of translational cancer research. Research efforts are mainly aimed at the evaluation and translation of the diagnostic and therapeutic potential of novel molecular targets involved in the development and progression of cancer by means of molecular imaging techniques to enhance patient care.
 

This includes (1) the design, synthesis and characterization of novel molecular probes (radiotracers), (2) the assessment and characterization of novel molecular targets, and (3) the application of novel technologies for molecular imaging and therapy of cancer.

 

Publications

List of publications (2005-2010):

V. R. Bouvet, M. Wuest, L. I. Wiebe, F. Wuest. Synthesis of hypoxia imaging agent 1-(5-deoxy-5-fluoro--D-arabinofuranosyl)-2-nitroimidazole [18F]FAZA using microfluidic technology. Nucl. Med. Biol. Submitted.
 
M. Wuest, B. J. Trayner, T. N. Grant, H.-S. Jans, J. Mercer, D. Murray, F. G. West, A. J. McEwan, F. Wuest, C. I. Cheeseman. Radiopharmacological evaluation of 6-deoxy-6-[18F]fluoro-D-fructose as a radiotracer for PET imaging of GLUT5 in breast cancer. Nucl. Med. Biol. Submitted.
 
M. Pretze, F. Wuest, T. Peppel, M. Köckerling, C Mamat. The Traceless Staudinger Ligation with Fluorine-18: A Novel and Versatile Labeling Technique for the Synthesis of Radiotracers for Positron Emission Tomography (PET). Tetrahedron Lett. Submitted.
 
I. Koslowsky, J. Mercer, F. Wuest. Synthesis and application of 4-[18F]fluorobenzylamine: A versatile building block for the preparation of PET radiotracers. Org. Biomol. Chem. In Press.
 
F. Graf, B. Mosch, L. Koehler, R. Bergmann, F. Wuest, J. Pietzsch. Cdk4/6 inhibitors: perspectives in cancer therapy and imaging. Mini Rev. Med. Chem. 2010, 10, 527-539.

S. Richter, T. Ramenda, R. Bergmann, T. Kniess, J. Steinbach, J. Pietzsch, F. Wuest. Synthesis and evaluation of phosphopeptide-neurotensin(8-13) dimers via click chemistry. Bioorg. Med. Chem. Lett. 2010, 20, 3306-3309.
 
L. Vogler, K. Gagnon, S. McQuarrie, F. Wuest. Iodine-124: A promising iodine radioisotope for positron emission tomography (PET). Molecules. 2010, 15, 2686-2718.
 
L. Koehler, F. Graf, R. Bergmann, J. Steinbach, J. Pietzsch, F. Wuest. Synthesis and radiopharmacological evaluation of 124I-labeled CDK4 inhibitors. Eur. J. Med. Chem. 2010, 45, 727-737.
 
F. Graf, L. Koehler, T. Kniess, F. Wuest, B. Mosch, J. Pietzsch. Cell cycle regulating kinase Cdk4 as a potential target for tumor cell treatment and tumor imaging. J. Oncol. 2009, 106378, 1-12.
 
T. Ramenda, T. Kniess, R. Bergmann, J. Steinbach, F. Wuest. Radiolabelling of proteins with fluorine-18 via click chemistry. Chem. Comm. 2009, 48, 7521-7523.
 
S. Richter, R. Bergmann, J. Pietzsch, T. Ramenda, J. Steinbach, F. Wuest. Fluorine-18 labeling of phosphopeptides: A potential approach for the evaluation of phosphopeptide metabolism in vivo. Biopolymers. 2009, 92, 479-488.
 
T. Kniess, R. Bergmann, M. Kuchar, F. Wuest. Synthesis of a potential tyrosine kinase inhibitor by Knoevenagel condensation of oxindole with 4-[18F]fluorobenzaldehyde. Bioorg. Med. Chem. 2009, 17, 7732-7742.
 
C. Mamat, A. Flemming, M. Köckerling, J. Steinbach, F. Wuest. Pd-catalyzed cross coupling of selected iodophenyl esters with HPPh2 for the synthesis of benzoate-functionalized phosphanes as novel building blocks for the traceless Staudinger Ligation. Synthesis. 2009, 19, 3311-3321.
 
F. Wuest, C. Hultsch, M. Berndt, R. Bergmann. Direct labelling of peptides with 2-[18F]fluoro-2-deoxy-D-glucose ([18F]FDG). Bioorg. Med. Chem. Lett. 2009, 19, 5426-5428.
 
E. von Guggenberg, J. Sader, J. Wilson, S. Shahhosseini, I. Koslowsky, F. Wuest, J. Mercer. Automated synthesis of an 18F-labelled pyridine-based alkylating agent for high yield oligonucleotide conjugation. Appl. Radiat. Isotop. 2009, 67, 1670-1675.
 
J. Pietzsch, F. Wuest. Fluorine-18 radiolabelling of native and oxidized proteins: An important tool in the pathophysiologists’ toolbox. Chemistry Today. 2009, 27, 12-14.
 
C. Mamat, T. Ramenda, F. Wuest. Application of click chemistry for the synthesis of radiotracers for molecular imaging. Mini Rev. Org. Chem. 2009, 6, 21-34.
 
F. Wuest, L. Vogler, M. Berndt, J. Pietzsch. Systematic Comparison of Two Novel Thiol-Reactive Prosthetic Groups for 18F Labeling of Peptides and Proteins with the Acylation Agent Succinimidyl-4-[18F]fluorobenzoate [18F]SFB. Amino Acids. 2009, 36, 283-295.
 
F. Wuest, L. Tang, T. Kniess, J. Pietzsch, M. Suresh. Synthesis and cyclooxygenase inhibition of various (aryl-1,2,3-triazole-1-yl)methanesulfonyl derivatives. Bioorg. Med. Chem. 2009, 17, 1146-1151.
 
F. Wuest, T. Kniess, B. Henry, B. W. Reeters, P. H. Wiegerinck, J. Pietzsch, R. Bergmann. Radiosynthesis and radiopharmacological evaluation of [N-methyl-11C]ORG 34850 as a glucocorticoid receptor ligand. Appl. Radiat. Isotop. 2009, 67, 308-312.
 
F. Wuest, M. Berndt, T. Kniess. Palladium-mediated cross-coupling reactions with [11C]methyl iodide and 4-[18F]fluorohalobenzenes for the synthesis of positron emission tomography (PET) radiotracers. Research Signpost. Recent Advances of Bioconjugation Chemistry in Molecular Imaging. X. Chen (Ed.). 2008, 155-173.

F. Wuest, T. Kniess, R. Bergmann, J. Pietzsch. Synthesis and evaluation in vitro and in vivo of a 11C-labeled cyclooxygenase-2 (COX-2) inhibitor. Bioorg. Med. Chem. 2008, 16, 7662-7670.
 
T. Kniess, M. Kuchar, F. Wuest. Facile synthesis of various nitro-substituted derivatives of Semaxinib (SU 5416). Synth. Commun. 2008, 38, 3017-3022.
 
F. Wuest, M. Berndt, R. Bergmann, J. Pietzsch. Synthesis and Application of [18F]FDG-maleimidehexyloxime ([18F]FDG-MHO): A [18F]FDG-based Prosthetic Group for the Chemoselective 18F-Labeling of Peptides and Proteins. Bioconjugate Chem. 2008, 19, 1202-1210.
 
B. Steiniger, T. Kniess, R. Bergmann, J. Pietzsch, F. Wuest. Radiolabeled glucocorticoids as molecular probes for imaging brain glucocorticoid receptors by means of positron-emission-tomography (PET). Mini Rev. Med. Chem. 2008, 8, 728-739.
 
J. Schlesinger, I. Közle, R. Bergmann, S. Tamburini, C. Bolzati, F. Tisato, B. Noll, S. Klussmann, S. Vonhoff, F. Wuest, H.-J Pietzsch, J. Steinbach. An 86Y-Labeled Mirror-Image Oligonucleotide: Influence of Y-DOTA Isomers on the Biodistribution in Rats. Bioconjugate Chem. 2008, 19, 928-939.
 
S. Richter, R. Bergmann, J. Pietzsch, B. Beuthien-Baumann, F. Wuest. Radiosynthesis of n.c.a. sodium [18F]fluoroacetate and radiopharmacological characterization in rats and tumour-xenografted mice. Current Radiopharmaceuticals. 2008, 1, 103-109.
 
T. Kniess, K. Rode, F. Wuest. Practical experiences with the synthesis of [11C]CH3I through gas phase iodination reaction using a TRACERlabFXC synthesis module. Appl. Radiat. Isotop. 2008, 66, 482-488.
 
F. Füchtner, J. Zessin, P. Mäding, F. Wuest. Aspects of 6-[18F]fluoro-L-DOPA preparation: Deuterochloroform as a substitute solvent for Freon 11. Nuklearmedizin. 2008, 47, 62-64.
 
F. Wuest, K. E. Carlson, J. A. Katzenellenbogen. Expeditious synthesis of steroids containing a 2-methylsulfanyl-acetyl side chain as potential glucocorticoid receptor imaging agents. Steroids. 2008, 73, 69-76.
 
F. Wuest, T. Kniess, J. Pietzsch, B. Henry, R. Bergmann. Synthesis and radiopharmacological characterization of [11C]AL-438 as a nonsteroidal ligand for imaging brain glucocorticoid receptors. Bioorg. Med. Chem. Lett. 2007, 17, 4035-4039.
 
F. Wuest, M. Berndt, K. Strobel, J. van den Hoff, X. Peng, J. L. Neumeyer, R. Bergmann.. Synthesis and radiopharmacological characterization of [18F]MCL-322 as a potential PET radiotracer for imaging dopamine transporter. Bioorg. Med. Chem. 2007, 15, 4511-4519.
 
T. Ramenda, R. Bergmann, F. Wuest. Synthesis of 18F-labeled neurotensin(8-13) via copper-mediated 1,3-dipolar [3+2]cycloaddition reaction. Lett. Drug Des. Discov. 2007, 4, 279-285.
 
C. Hultsch, M. Berndt, R. Bergmann, F. Wuest. Radiolabelling of multimeric neurotensin(8-13) analogues with the short-lived positron emitter fluorine-18. Appl. Radiat. Isotop. 2007, 65, 818-826.
 
S. Gester, J. Pietzsch, F. Wuest. Synthesis of 18F-labelled stilbenes from 4-[18F]fluorobenzaldehyde using the Horner-Wadsworth-Emmons reaction. J. Label. Compd. Radiopharm. 2007, 50, 105-113.
 
M. Berndt, J. Pietzsch, F. Wuest. Labeling of low-density lipoproteins using the 18F-labeled thiol-reactive reagent N-[6-(4-[18F]fluorobenzylidene)aminooxyhexyl]-maleimide [18F]FBAM. Nucl. Med. Biol. 2007, 34, 5-15.
 
F. Wuest, M. Berndt, T. Kniess. Carbon-11 labelling chemistry based upon [11C]methyl iodide. Ernst Schering Research Foundation Workshop , Vol. 62; Schubiger, P.August; Lehmann, Lutz; Friebe, Matthias (Eds.). 2006, 183-213.

F. Wuest. Fluorine-18 labelling of small molecules: The use of 18F-labelled aryl fluorides derived from no-carrier-added [18F]fluoride as labelling precursors. Ernst Schering Research Foundation Workshop, Vol. 62; Schubiger, P.August; Lehmann, Lutz; Friebe, Matthias (Eds.). 2006, 51-78.
 
H. M. Bigott, R. Laforest, X. Liu, A. Ruangma, F. Wuest, M. J. Welch. Advances in the Production, Processing and MicroPET Image Quality of Technetium-94m. Nucl. Med. Biol. 2006, 33, 923-933.
 
J. Schlesinger, R. Bergmann, S. Klussmann, F. Wuest, H.-J. Pietzsch, J. Steinbach. 86Y-Labelled L-RNA Oligonucleotides as Molecular Probes for Positron Emission Tomography (PET). In: Technetium, Rhenium and Other Metals in Chemistry and Nuclear Medicine 7. M. Mazzi, Ed., SGEditoriali, Padova. 2006, 211-214.
 
B. Steiniger, F. Wuest. Synthesis of 18F-labelled biphenyls via Suzuki cross-coupling with 4-[18F]fluoroiodobenzene. J. Label. Compd. Radiopharm. 2006, 49, 817-827.
 
C. Hultsch, B. Pawelke, R. Bergmann, F. Wuest. Synthesis and evaluation of novel multimeric Neurotensin (8-13) analogues. Bioorg. Med. Chem. 2006, 14, 5913-5920.
 
J. Schlesinger, R. Bergmann, S. Klussmann, F. Wuest. Synthesis and Radiopharmacological Characterisation of 86Y- and 68Ga-Labelled L-RNA Oligonucleotides as Molecular Probes for Positron Emission Tomography (PET). Lett. Drug Des. Discov. 2006, 6, 330-335.
 
P. Mäding, J. Zessin, U. Pleiß, F. Füchtner, F. Wuest. Synthesis of a 11C-labelled taxane derivative by [1-11C]acetylation. J. Label. Compd. Radiopharm. 2006, 49, 357-365.
 
F. Wuest, M. Berndt. 11C-C bond formation by palladium-mediated cross-coupling of alkenylzirconocenes with [11C]methyl iodide. J. Label. Compd. Radiopharm. 2006, 49, 91-100.
 
J. Zessin, W. Deuter-Conrad, M. Kretzschmar, P. Brust, J. Steinbach, F. Wuest, B. Pawelke, R. Bergmann. [11C]SMe-ADAM, an imaging agent for the serotonin transporter: Synthesis, pharmacological characterization and microPET studies in rats. Nucl. Med. Biol. 2006, 33, 53-63.
 
F. Wuest. Radiopharmakaforschung: Trends und neue Konzepte. Der Nuklearmediziner. 2005, 4, 206-214.
 
J. Pietzsch, R. Bergmann, F. Wuest, B. Pawelke, C. Hultsch, J. van den Hoff. Catabolism of native and oxidized low density lipoproteins (LDL): in vivo insights from small animal positron emission tomography studies. Amino Acids. 2005, 29, 389-404.
 
C. Hultsch, R. Bergmann, B. Pawelke, J. Pietzsch, F. Wuest, B. Johannsen, T. Henle. Biodistribution and catabolism of 18F-labelled isopeptide N-(-glutamyl)-L-lysine. Amino Acids. 2005, 29, 405-413.
 
S. Gester, F. Wuest, B. Pawelke, R. Bergmann, J. Pietzsch. Synthesis and biodistribution of a 18F-labelled resveratrol derivative for small animal positron emission tomography (PET). Amino Acids. 2005, 29, 415-428.
 
F. Wuest. Aspects of positron emission tomography (PET) radiochemistry as relevant for food chemistry. Amino Acids. 2005, 29, 323-339.

J. Pietzsch, R. Bergmann, F. Wuest, J. van den Hoff. In vivo metabolism of oxidized low density lipoproteins:Insights from small animal positron emission tomography (PET) studies. Recent Res. Devel. Mol. Cell. Biochem. 2005, 2, 153-177.
 
P. Mäding, F. Füchtner, F. Wuest. Modul-assisted synthesis of the labelling agent [18F]SFB. Appl. Radiat. Isotop. 2005, 63, 329-332.

F. Wuest, T. Kniess, M. Kretzschmar, R. Bergmann. Synthesis and biodistribution of a 18F-labelled corticosteroid as ligand for mapping brain glucocorticoid receptors by means of PET. Bioorg. Med. Chem. Lett. 2005, 15, 1303-1306.
 
F. Wuest, A. Höhne, P. Metz. Synthesis of 18F-labelled COX-2 inhibitors via Stille reaction with 4-[18F]fluoroiodobenzene. Org. Biomol. Chem. 2005, 3, 503-507.
 
F. Wuest, T. Kniess. N-arylation of indoles with 4-[18F]fluoroiodobenzene Synthesis of 18F-labelled 2 receptor ligands for positron emission tomography (PET). J. Label. Compd. Radiopharm. 2005, 48, 31-43.
 
R. Garcia, C. Xavier, A. Paulo, I. Santos, T. Kniess, R. Bergmann, F. Wuest. Synthesis and Biological Evaluation of S-[11C]Methylated Mercaptoimidazole Piperazinyl Derivatives as Potential Radioligands for Imaging 5-HT1A Receptors by Positron Emission Tomography (PET). J. Label. Compd. Radiopharm. 2005, 48, 301-315.